Process for introducing nucleic acids and other biologically active in the nucleus of eukaryotic cells using higher electric current molecules.

Process for introducing nucleic acids, peptides, proteins and / or other biologically active molecules in the cell nucleus of eukaryotic cells using electric current, transmitting at least one voltage pulse to the cells, wherein the charge quantity is measured emitted by the voltage pulse in at least one selectable time interval, performing a comparison of a theoretical amount of charge previously adjusted with the actual amount of charge emitted, and that upon reaching or exceeding the amount of theoretical load momentum is interrupted tension.