Using Canalography to Visualize the In Vivo Aqueous Humor Outflow Conventional Pathway in Humans

Amicrocatheter filled with 1.25 μg of indocyanine green (ICG) diluted in 1 mL of viscoelastic solution was placed into the Schlemm canal (SC) to enlarge the drainage canal; the dye was then injected into the SC. Near-infrared fluorescence real images of aqueous humor outflow structures in in vivo human eyes were obtained. A, Introduction of the flexible microcatheter into the lumen of the SC. B, Infrared picture at the beginning of ICG injection. C, After injection of ICG plus viscoelastic, visualization of the SC, collector channels, and 3 aqueous veins (arrowheads). The fourth aqueous vein is masked by themicrocatheter. D, Higher-magnification image showing in detail the aqueous vein morphology. Themethod is simple and safe andmakes possible the real visualization of the aqueous humor outflow system during canaloplasty. Proteomic Landscape of the Choroid-RPE Complex Original Investigation Research