[Tumor Associated Fibroblasts Promote PD-L1 Expression in Lung Cancer Cells].

: 背景与目的 肿瘤相关成纤维细胞（tumor-associated fibroblasts, TAF）是肿瘤微环境的重要组成部分，可抑制免疫细胞的功能。在肿瘤免疫中CD8+T细胞发挥重要的作用，T细胞膜表面程序性死亡因子1（programmed death factor 1, PD-1），与其配体PD-L1（programmed death factor ligand 1, PD-L1）结合对T细胞的激活起负调节作用。本研究旨在探讨TAF对肺癌细胞PD-L1表达的影响。方法 我们以肺癌细胞株H1975、H520和TAF细胞进行Transwell非接触式共培养48 h的H1975、H520细胞为实验组，单独培养的H1975、H520细胞为对照组，两组培养条件一致。倒置显微镜计数实验组和对照组H1975、H520细胞数、流式细胞仪分别检测实验组和对照组肺癌细胞PD-L1的蛋白表达率、RT-PCR分别检测实验组和对照组肺癌细胞PD-L1 mRNA的表达。结果 每100 μm2细胞计数，H1975细胞实验组为（46±21）个，对照组为（16±5）个（P<0.05）；H520细胞实验组为（38±10）个，对照组为（12±5）个（P<0.05）。PD-L1蛋白表达率，H1975细胞实验组为（20.93%±3.54%），对照组为（12.58%±2.52%）（P<0.05）；H520细胞实验组（19.26%±3.04%），对照组为（11.60%±2.65%）（P<0.05）。mRNA表达水平，H1975细胞实验组为（16.45±1.25）pg/mL，对照组为（7.78±1.27） pg/mL（P<0.05）；H520细胞实验组为（15.38±2.02）pg/mL，对照组为（7.20±1.58）pg/mL（P<0.05）。结论 TAF促进肺癌细胞株H1975、H520的生长，增强细胞株PD-L1表达。. METHODS: Lung cancer cell lines H1975 and H520 were co-cultured with (experiment) or without TAF (control) via Transwell assay for through 48 hours under the same culture condition. H1975 and H520 cells were counted using a microscope. The protein and mRNA expression levels of PD-L1 were detected by FCM assay and PCR analysis, respectively. RESULTS: The numbers of lung cancer cells in 100 μm2 for H1975 and H520 cells are (46±21) and (38±10) in the experiment group, respectively, and (16±5) and (12±5) in the control group, respectively (P<0.05). The expression levels of the PD-L1 protein in H1975 and H520 cells are (20.93%±3.54%) and (19.26%±3.04%) in the experiment group, respectively, and (12.58%±2.52%) and (11.60%±2.65%) in the control group, respectively (P<0.05). The mRNA expression levels in H1975 and H520 cells are (16.45±1.25) and (15.38±2.02) pg/mL in the experiment group, respectively, and (7.78±1.27) and (7.20±1.58) pg/mL (P<0.05) in the control group, respectively (P<0.05). CONCLUSIONS: TAF promotes the growth and increases the expression of PD-L1 in H1975 and H520 cells.
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