BMR Microbiology-Antibacterial Evaluation And Phytochemical Screening Of Leaves And Fruits Of Passiflora subpeltata Ortega

The purpose of present work is to study antimicrobial activity and medicinally active principles present in different solvent extracts obtained from leaves and fruits of Passiflora subpeltata.The active principles were isolated by Soxhlet extractor and identified by preliminary phytochemical test. The Soxhlet extraction was performed using petroleum ether, chloroform, ethyl acetate and methanol. The results of analyses of solvent extract confirmed the active substances were sterols, glycosides, flavonoids, tannins. The antibacterialia tests of isolated substances were performed with pathogenic bacteria like – Escherichia coli , Klebsiella pneumoniae, Enterobacter aerogenes, Staphylococcus aureus, and Enterobacter faecalis. The observation of microbial test of methanol and ethyl acetate extract of both leaves and fruits supports to antibacterial activity to the greater extent than petroleum ether, and chloroform extract. In this study, both Gram positive and negative bacteria tested were found to be sensitive to methanol and ethyl acetate extract of both leaves and fruits. P.subpeltata revealed the highest antibacterial activity at a minimum inhibitory concentration against E.aerogenes, S.aureus, and E.faecalis. These findings suggest the need for further research of this herb and its products. The results provide justification for the use of the P.subpeltata in folk medicine to treat various infectious diseases.