Influence of miR-21 on osteogenic capability in human bone marrow mesenchymal stem cells in patients with postmenopausal osteoporosis

Objective To investigate the osteogenic capability of miR-21 in human bone marrow mesenchymal stem cells in patients with postmenopausal osteoporosis (PMOP-hBMMSCs).Methods The human bone marrow mesenchymal stem cells (hBMMSCs) and PMOP-hBMMSCs were isolated and cultured.The osteogenic capabilities of these two kinds of cells were compared.After over-expression miR-21,the osteogenic potential was analysed in PMOP-hBMMSCs.The expression of Runx2 and Osterix,genes in H-hBMMSCS and PMOP-hBMMSCs were respectively detected by real time RT-PCR and western blot.Results The expression level of miR-21 was higher,ALP was more active,and the calcium nodules staining area was larger in the PMOP-hBMMSCs group than in the PMOP group(P ＜ 0.05).Up-regulation was found in the expressions of Runx2 and Osterix mRNA and protein of Over-expression of miR-21 PMOP-hBMMSCs compared to PMOP(P ＜ 0.05,P ＜ 0.01).Conclusion After the transfection of miR-21,the osteogenic ability of PMOP-hBMMSCs can be enhanced. Key words: miR-21;  PMOP-hBMMSCs;  Transfection