Protective effect of β ecdysone on rat heart transplantation

OBJECTIVE: To explore whether β ecdysone(EDS) has some protective effect on reperfusion in rat heart transplantation. METHODS: Model of rat ectopic heart transplantation was made by transplant donor heart to peritoneal cavity of receptor. EDS (ig, 20mg·kg -1 , 1/d,use 7d) was administrated to heart transplantation rat. Observation was made on the items of lactate dehydrogenase (LDH), glutamic oxaloacetic transminase (GOT),creating phosphokinase (CPK) inserum. Malondialdehyde(MDA) in serum was measured by radioimmunoassay acid fluorescence methods. Superoxide dismutase (SOD) in serum was determined by radioimmuoassay. Pathological change of myocardium especially mitochondrion was detected by transmission electron microscope. RESULTS: EDS is able to decrease lipid oxidation action in rat heart transplantation, cause to reduce the release of myocardium enzymogram and abate the pathological changes of mitochondrion. CONCLUSION: EDS may have some protective effect of reperfusion injury in rat heart transplantation. The mechanism of protective effect may be related to EDS's property of anti lipoperoxidation.