Boundary Lubrication, Hemifusion, and Self-Healingof Binary Saturated and Monounsaturated Phosphatidylcholine Mixtures

A wide range of phosphatidylcholine (PC) lipids with different degrees of unsaturation has been identified in the human synovial fluid and on the cartilage surface. The outstanding lubricity of the articular cartilage surface has been attributed to boundary layers comprising complexes of such lipids, though to date only lubrication by single-component PC-lipid-based boundary layers has been investigated. As distinguishable lubrication behavior has been found to be related to the PC structures, we herein examined the surface morphology (on mica) and the lubrication ability of binary PC lipid mixtures, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), using atomic force microscopy (AFM) and a surface force balance (SFB). These two PC lipids are among the most abundant saturated and unsaturated PC components in synovial joints. Small unilamellar vesicles (SUVs) prepared from DPPC-POPC mixtures (8:2, 5:5, and 2:8, molar ratios) ruptured and formed ...