Effects of celecoxib combined with p65miRNA on tumor growth in breast cancer xenografts in nude mice

Objective To evaluate the effects of celecoxib combined with p65miRNA on tumor growth in breast cancer xenografts in nude mice and its mechanism.Methods After successfully establishing breast cancer xenografts in nude mice,the mice were randomly divided into control,the neg-miRNA,the p65 miRNA,the celecoxib,the celecoxib combined with neg-miRNA and the celecoxib combined with p65miRNA groups.On the 42nd day after treatment,tumor volume,weight and the rate of tumor inhibition were measured.Tumor tissues were collected and assessed for the detection of p65,VEGF and MMP-2 by immunohistochemistry,Western blotting and RT-PCR.Results The rate of tumor inhibition was 43.23% in the celecoxib group,40.72% in the p65miRNA group and 61.69% in the celecoxib combined with p65miRNA group.Immunohistochemical results showed a significant decrease in p65 expression of the p65miRNA group.Western blotting and RT-PCR analysis showed that the expressions of VEGF and MMP-2 were significantly reduced in the celecoxib,the p65miRNA and the celecoxib combined with p65miRNA groups.Conclusions The combination of celecoxib and p65miRNA can enhance the anti-tumor effect in human breast cancer xenografts,by down-regulating expressions of VEGF and MMP-2.