Sequencing and Phylogenetic Analysis of the 16S rDNA of Ralstonia solanacearum MR111 in Mulberry

PCR amplification and sequence analysis was applied to 16S rDNA of strain MR111 isolated from diseased mulberry.The results showed that the 1 435 bp of 16S rDNA sequence was obtained from PCR amplification.The results of BLAST on NCBI indicated that the 16S rDNA has 95% identity with Ralstonia solanacearum strain PC-3 and RSGC-1(GenBank accession number: HM992931 and JQ320374).The results of phylogenetic analysis based on the 16S rDNA from 28 Ralstonia solanacearum strains showed that MR111 is in the same branch with GY0501 from Ipomoea aquatica and ZCz-3 from Lycopersicon esculentum.Therefore,the MR111 is identified as Ralstonia solanacearum and named as MR111a.