Isolation of acid blue B-degrading Fusarium sp.HJ01 and the studies on its degradation characteristics

An acid blue B-degrading strain (Fusarium sp.)HJ01 was isolated from bentonite. The growing characteristics and kinetics, the characteristics of enzymatic biodegradation of Fusarium sp. HJ01 were analyzed. And the effects of biomass on decolorization of acid blue B in aqueous samples were reported. The results showed that the pH range between 6 and 7 for growth of Fusarium sp. HJ01 was feasible, and the sucrose and NH_4Cl,is also feasible for carbon source and nitrogen source respectively. The growth kinetics of Fusarium sp. HJ01 was fitted by the modified logistic law model. The molecular weight of laccase from Fusarium sp. HJ01 was approximately 66kD analyzed by SDS-PAGE, and laccase activity was up to from 431?U·mg~(-1)·s~(-1) to 812?U·mg~(-1)·s~(-1) at the condition of low nitrogen source. The Fusarium sp. HJ01 incubated for 96?h at 25 ℃ were added to the liquid medium containing acid blue B (100?mg·L~(-1)). And when cultured continuously for another 96?h, the decolorization rate of acid blue B was up to 100%. The ultraviolet-visible spectrum showed that the chromophore anthraquinone ring of acid blue B was broken down by laccase.