Seed Germination of Picea crassifolia Kom

Seeds were collected from the natural population of Picea crassifolia Kom.in Tianzhu County,Wuwei City,Gansu Province,China in November 2010.Seed vigor was tested by TTC,the experiments were conducted to investigate the response of seed germination to temperature,light,moisture stress and salinity stress,and some references were finally provided for transplanting P.crassifolia Kom.from Tianzhu to Minqin County.The results are as follows: ① The vigor of P.crassifolia Kom.seeds was 72%;② Under a complete dark,seeds could not germinate when temperature was lower than 10 ℃,and the optimal temperature for germination was 25/15 ℃(72%);germination rate of P.crassifolia Kom.seeds under the 24-hour complete dark and 24-hour light were significantly higher than that under 14-hour light and 10-hour dark;③ Seed germination could be improved when NaCl concentration was 50 mmol·L-1,but it could be restricted when NaCl concentration was higher than 50 mmol·L-1;seed germination could be recovered after salinity stress was removed,the recovery germination rate(RGR) was increased at first and then decreased with the increase of NaCl concentration,and RGR reached its maximum(13%) when NaCl concentration was 400 mmol·L-1;④ Seed germination was significantly decreased with the increase of PEG-6000 osmotic potential,and seeds could not germinate when the osmotic potential was-0.9 MPa;after PEG-6000 stress was removed,RGR was increased at first and then decreased with the increase of PEG-6000 concentration,and it reached its maximum(35%) when the osmotic potential was-0.9 MPa.