An anti-biofilm material: polysaccharides prevent the precipitation reaction of silver ions and chloride ions and lead to the synthesis of nano silver chloride.

The formation of biofilm is one of the causes of bacterial pathogenicity and drug resistance. Recent studies have reported a variety of anti-biofilm materials and achieved good results. However, it is still very important to develop some materials with wider application scenarios or higher biofilm resistance. In this study, a new method to rapid synthesize nano silver chloride with anti-biofilm activity is proposed. The bacterial extracellular polysaccharides are used to adsorb silver ions and chloride ions and inhibit the generation of silver chloride precipitation, and then the mixed solution is induced by ultraviolet light to quickly synthesize nano silver chloride in situ. This method is generalizable. A variety of polysaccharides can be used to synthesize nano silver chloride. The novel complex was characterized by XRD, UV-Vis and TEM methods, and further tested the bactericidal ability and anti-biofilm activity. the results showed that our novel nano sliver chloride could effectively destroy the bacteria in biofilm at a bio-safe concentration. In view of the high stability of nano sliver chloride, we propose that the novel material has the potential as a long-term antibacterial material.