Hydrogen sulfide induces miR194 in diabetic nephropathy and mitigates renal fibrosis (689.10)

Introduction: Recent evidences suggest that hydrogen sulfide (H2S) has a therapeutic potential for several clinical complications including renovascular fibrosis. Similarly, micro RNAs (miRs) has also gained substantial biomedical research interest with relevance to renal fibrosis. In this regard miRs control reactive oxygen species (ROS) and high level of ROS persuades poly-ADP-ribose-polymerase-1 (PARP1) which induces extracellular matrix (ECM) associated with vascular fibrosis. Among miRs, miR194 is prevalent in the mammalian kidney; however, its role in diabetic nephropathy and whether H2S has any potential implication on miRs regulation associated with diabetic renal fibrosis is unknown. Hypothesis: The objective of this study was to determine whether miR194 has potential roles in diabetic nephropathy which leads to PARP 1 activation by ROS induction causing renal fibrosis. Materials and methods: C57BL/6J (Wild type, WT) and diabetic (Akita, C57BL/6J-Ins2Akita) mice were used in this study. Animals w...