Resveratrol biosynthesis in vitro culture conditions on grapevine (Feteasca Neagra and Cabernet Sauvignon) under the action of AlCl3 as elicitor agent.

Stillbenes are considered the most important phytoalexin group synthesized in the grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main typical stillbenes of grapevine, which show antifungal and pharmaceutical characteristics, are resveratrol and his derivatives. Recent studies highlight their benefit for the human health by their antifungal, anticarcinogen, hypolipidemic and antidiabetic properties, besides their already known fungitoxic characteristics. The present study focused upon the induction of resveratrol biosynthesis on in vitro vine culture conditions under the effect of AlCl3 as eliciting agent. The specific medium for vine multiplication (MS 0.03%; 0.05%). The resveratrol dosage was performed through the high performance liquid chromatography (HPLC), using a Merck - Lunchrom pressure liquid chromatograph and a UV detector. A more intensive resveratrol biosynthesis has been observed in the Cabernet Sauvignon variety, where the synthesized resveratrol quantity in the plant was up to 97.94 µg/g d.m. with a 0.05 % AlCl3 concentration in the culture medium.