Detection of Antibodies against Duck Plague Virus by ELISA

An indirect enzyme_linked immunosorbent assay(ELISA)was established as following method.The crude extraction of plague virus was purified as coating antigen by means of sephadex_G200 chromatography.The goat was immunized by negative antisera from the ducks free from DPV for goat IgG against duck,which was labelled with horseradish peroxidase(HRP)by Nalo 4.Maternal antibodies and adaptive immune antibodies from duckling and the sera sample from the ducks in different clinical immune Qtatus were examined by ELISA.The results showed that the method is high specificity and sensitivity for detection of antibodies against DPV,and can be used in clinical serological surveillance.