Establishment of Bovine Viral Diarrhea Virus Recombinant Protein ELISA Assay

【Objective and Method】The recombinant expressing plasmid PET-(1-345),which include the foreign gene fragment coding the main antigenic of bovine viral diarrhea virus(BVDV),was transformed into host cell BL21(DE3) and induced with IPTG..West-Blot assay reactivity and specificity of the target protein.Then, the target protein,after being purified with the his band kit,was used as an antigen in the square titration test and optimal dilution rate for the ELISA was confirmed.【Result】The recombinant protein expression can reach 19.7% in total amount of somatic protein.West-Blot assay showed the good immunological reactivity and specificity of the target protein,43 bovine sera samples were detected with the indirect ELISA assay.The result showed that the consistancy rate between the result detected the bovine sera sample and the result detected with imported agent is 93.55%.【Conclusion】Bovine viral diarrhea virus recombinant protein E2 ELISA assay is a practicable method.