Comparative analysis of nucleotide sequence 16S rRNA of Eperythrozoon suis of three provinces in northeast

A systematic experiment was conducted on the farm of Henan Agricultural University,Zhengzhou,China.Using Yunyan 85 planted in 400mm×340mm plastic pots,treatments were layed out with 5levels of 0(CK),200,400,600 and 800mg/kg(added as fulvic acid)in soil with 50mg/kg sodium arsenate(Na3AsO4·12H2O),respectively,to determine the effect on nitrogen metabolism of flue-cured tobacco in fulvic acid applied to arsenic-contaminated soil.The results showed that different contents of fulvic acid had different effects on nitrogen metabolism of flue-cured tobacco.Although activity of glutamate-pyruvate transaminase(GPT)was increased in fulvic acid applied to arseniccontaminated soil in the rosette stage,but finally improved NO3--N assimilation and inhibited NH4+-N assimilation.Reasonable content of fulvic acid decreased in free amino acids content,and in activity of glutamate-pyruvate transaminase(GPT)and asparaginase(ASG),which finally improved transformation of nitrogen of flue-cured tobacco.Fulvic acid increased protein content after budding stage and nicotine content during the whole growing period,but decreased protein content at rosette stage.The best treatment in this experiment was that applied 600mg/kg of fulvic acid to arsenic-contaminated soil.