Cyclic regulation of B220 antigen expression in immature B cell lines

Three independent immature B cell lines transformed with temperature-sensitive mutants of Abelson murine leukaemia virus (A-MuLV) persistently expressed B220 antigen detected by both of the anti-B220 antibodies RA3-6B2 and 14. 8 during culture at the permissive temperature (3 5°C). RA3-6B2 recognizes the B220 epitope and that 14.8 is a CD45RA antibody. However, when the culture temperature was shifted up to the non-permissive temperature (39°C) and the culture was continued for 2-3 weeks, a population of the cells (RA3-6B2 − 14.8 + cells) that lost RA3-6B2 antigen appeared in all three cell lines. From these populations, RA3-6B2 − 14.8 + subclones were independently isolated by limiting dilution and the phenotype was stable during culture at the permissive temperature