The Combined Application of Low Molecular Dextran, Papaverine and Low Molecular Weight Heparin Sodium to Improve Testicle Survival Rate after the Detorsion of Testicular Torsion in Rabbits

Objective: To observe the effect of low molecular dextran, papaverine and low molecular weight heparin sodium on the blood flow after detorsion of the testicular torsion in rabbits. Methods: 36 male rabbits(weighing 1.8-2.4 kg, 10-14 months of age) were randomly divided into experimental group and control group, 18 in each group. Produced by surgical testicular torsion model (720° counterclockwise rotation of the left side testicle), every rabbit was accepted surgery detorsoin after 9 hours and found all twisted testis were dark. After fomentation with warm salt water, these testis were observed no significant improvement in colors, then retained and fixed all these testis. Low molecular dextran(5 ml/kg, ear marginal vein injection, 1/d), papaverine(1.5 mg/kg, intramuscular, 3/d) and low molecular weight heparin sodium(200 IU/kg, subcutaneous, 1/d) were applied to promote the recovery of testicular blood flow for 5 days in experimental group, and penicillin(4000 U/kg, intramuscular, 1/d) for 7 days. Control group was only given penicillin(4000 U/kg, intramuscular, 1/d) to prevent wound infection for 7 days. Then Color Doppler ultrasound was used to observe the testicular blood flow siganals that were divided into 0, 1, 2, 3 classes. Class 2 and class 3 could be considered as testicular survival. Opened scrotum, according to color, texture of testicle and bright bleeding, to determine whether the testicle was alive or not. If testicle was ruddy, flexible, and bright bleeding was detected, could prove testicle survival. Results: In experimental group, the testicular blood flow siganals: 0 class 4 cases, 1 class 3 cases, 2 class 4 cases, 3 class 7 cases. In control group, the testicular blood flow siganals: 0 class 9 cases, 1 class 2 cases, 2 class 4 cases, 3 class 3 cases. Surgical exploration found 11 cases of testicle survival in experimental group and 7 cases in control group, which was significantly difference (p-0.05). Conclusions: The combined application of low molecular dextran, papaverine, low molecular weight heparin sodium can promote the recovery of testicular blood flow after detorsion of testicular torsion and improve testicle survival rate.