HPLC characteristic chromatogram of Sarcandrae Herba water extract and its preparations.

Objective To develop an HPLC characteristic chromatogram of Sarcandrae Herba water extract for evaluation of its quality and the related products.Methods The method was developed on a C18 column(250 mm×4.6 mm,5 μm) by gradient elution with acetonitrile-water(both containing 0.1% formic acid) as mobile phase at a flow rate of 1.0 mL/min with the detected wavelength at 330 nm and column temperature at 30 ℃.The quality of the water extract and its related products were evaluated by comparing with the established reference characteristic chromatogram.Results The HPLC characteristic reference chromatogram was established with good stability,precision,and reproducibility.Its similarity among 12 batches of the extract and 20 batches of related preparations ranged in 0.883-0.987 and 0.797-0.982,respectively.Furthermore,eight peaks in the characteristic chromatogram were identified as neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,β-hydroxypropiovanillone,caffeic acid,isofraxidin,rosmarinic acid-4-O-glucoside,and rosmarinic acid,respectively.Conclusion With anti-inflammatory bioactive ingredients and characteristic components included,the HPLC characteristic chromatogram reveales the profile of multiple chemical substances in Sarcandrae Herba water extract and its products and this would contribute to its effective quality control.