PD09-02 IMPLANTATION OF URINE-DERIVED STEM CELL FOR CHRONIC KIDNEY DISEASE INDUCED BY NEPHROTOXIC DRUG IN A RAT MODEL WITH RENAL ISCHEMIA

INTRODUCTION AND OBJECTIVES:Drug-induced nephrotoxicity often occurs in the patients with existing renal dysfunction or renal ischemia, potentially leading to chronic kidney disease (CKD). Prompt treatment of drug induced adverse effects is critical to prevent progression to end-stage renal disease. The goal of this study was to demonstrate the therapeutic potential of urine-derived stem cells (USC) to treat chronic kidney disease-induced by nephrotoxic drug and renal ischemia.METHODS:Human USC were collected, expanded and identified by flow cytometry analysis. The CKD model was induced by creating an ischemia-reperfusion injury followed by gentamicin injection. Twenty-eight immune deficient rats were divided into three groups: phosphate-buffered saline (PBS)-treated group (n=9), USC-treated group (n=9), and sham group with age-matched controls (n=10). Cell suspension of USC or PBS was bilaterally injected into the renal parenchyma (50x10^6/100µl/kidney) 9 weeks after the CKD model creation. Blood and uri...