Early Tissue Reaction After Second-Generation Cryoballoon Ablation Evaluated with Intracardiac Echocardiography: Evidence of Acute Tissue Edema After Cryoballoon Ablation

: Radiofrequency energy applications immediately produce tissue edema. This study aimed to investigate the acute tissue reaction immediately after second-generation cryoballoon applications using 3-dimensional intracardiac echocardiography (ICE) imaging technology.This study consisted of 10 patients with paroxysmal atrial fibrillation who underwent pulmonary vein isolation (PVI) using second-generation cryoballoons. Ablation was performed with a single 3-minute freeze strategy and exclusively 28-mm balloons. The left atrial and right pulmonary vein (PV) antra geometries were created with 3-dimensional ICE technology before and immediately after the PVI.Out of 20 right PVs, 19 were isolated exclusively with cryoballoons, and one right inferior PV (RIPV) required touch-up ablation. All 10 right superior PVs (RSPVs) were isolated by single cryoballoon applications, and RIPVs were isolated by a mean of 1.2 ± 0.4 applications. The total application time was 171 ± 19 and 203 ± 71 seconds, and nadir balloon temperature was -56.0 ± 4.9 and -53.8 ± 5.4°C for the RSPVs and RIPVs, respectively. In all patients, diffuse wall thickening of the antra and ostium of the right PVs was observed as compared to baseline. The wall thickening was 0-0.25 mm in 3 patients, and 0.25-0.5 mm in the remaining 7. During the median follow-up of 13 [10.2-17.2] months, 8 (80%) patients were free from arrhythmia recurrences. Nine (90%) patients underwent repeat cardiac computed tomography at a median of 6.0 [4.5-12.0] months after the initial procedure, and no PV stenosis was observed in all.Tissue edema and wall thickening appeared in the human left atrium immediately after second-generation cryoballoon ablation.