Miniscale preparation for large plasmid DNA from Bacillus thuringiensis

Miniprep of low copy number and large plasmid was done from three subspecies of Bacillus thuringiensis.The plasmid was purified using PEG 6000 instead of phenol and chloroform.Experiments demonstrate that consequences from this protocol were stable,the quality and yield of plasmid DNA produced from it have met the standard for many molecular biology experiments.