Plant Chemical Biology

Preface vii Contributors ix part one Introduction 1 1.1 From Herbal Remedies to Cutting-Edge Science: A Historical Perspective of Plant Chemical Biology 3 Michelle Q. Brown, Abel Rosado, and Natasha V. Raikhel part Two Sources of small molecules 19 2.1 Compound Collections 21 Reg Richardson 2.2 Combinatorial Chemistry Library Design 40 Robert Klein and Stephen D. Lindell 2.3 Natural Product-Based Libraries 64 Alan L. Harvey part three Identification of new chemical tools by High-Throughput Screening 73 3.1 Assay Design for High-Throughput Screening 75 Frank W. An and Jose R. Perez part four Use of chemical biology to study plant physiology 93 4.1 Use of Chemical Biology to Understand Auxin Metabolism, Signaling, and Polar Transport 95 Ken-ichiro Hayashi and Paul Overvoorde 4.2 Brassinosteroids Signaling and Biosynthesis 128 Takeshi Nakano and Tadao Asami 4.3 Chemical Genetic Approaches on ABA Signal Transduction 145 Eunjoo Park and Tae-Houn Kim 4.4 Jasmonic Acid 160 Christian Meesters and Erich Kombrink 4.5 Chemical Genetics as a Tool to Study Ethylene Biology in Plants 184 Yuming Hu, Filip Vandenbussche, and Dominique Van Der Straeten part five Use of chemical biology to study plant cellular processes 203 5.1 The Use of Small Molecules to Dissect Cell Wall Biosynthesis and Manipulate the Cortical Cytoskeleton 205 Darby Harris and Seth DeBolt 5.2 The Use of Chemical Biology to Study Plant Cellular Processes: Subcellular Trafficking 218 Ash Haeger, Malgorzata GBPangowska, and Stephanie Robert part six Target identification 233 6.1 Target Identification of Biologically Active Small Molecules 235 Paul Overvoorde and Dominique Audenaert part seven Translation of plant chemical biology from the lab to the field 247 7.1 Prospects and Challenges for Translating Emerging Insights in Plant Chemical Biology into New Agrochemicals 249 Terence A. Walsh 7.2 In Vitro Propagation 263 Hans Motte, Stefaan Werbrouck, and Danny Geelen Index 289