Study of prevention and treatment effects of baicalin on model Wistar rats with chronic obstructive pulmonary disease

Objective To study prevention and treatment effects of baicalin in model rats lung with chronic obstructive pulmonary disease(COPD) by COPD model rats.Methods According to the principle of random,25 Wistar rats (male) were divided into five groups,free group (group F),model contrast group (group C),maximum dose baicalin,middle dose and minimum dose treatment groups (group T1,group T2,group T3).Model rats were established by intratracheal instillation of lipopolysaccharide twice and exposed to cigarette smoke.In the process of model establishment,baicalin was given for treatment.After treatment,the rats were killed to study the pathological change of lung tissue with HE staining in each group,and measure mean alveolar number (MAN),the ratio of dried lung weight to fresh lung weight (RDW),immunohistochemical stains were performed to detect the expression of TNF-a.Results The COPD rats model was successfully made.The RDW of group F was 15.36±0.43,group C 10.86±0.51,group T1 13.86± 0.44,group T2 13.16±0.42,group T2 12.24±0.36.The MAN of group F was 99.00±5.92,group C 40.00± 5.93,group T1 65.00±5.15,group T2 62.00±6.32,group T3 58.00±4.47,TNF-a immunohistochemical stains were performed,group F was 137.11±3.26,group C 58.75±1.40,group T1 113.92.00±4.62,group T284.83±5.32,group T3 65.43±5.85.Conclusions Baicalin has some effects on COPD model rats.There is some outlook on baicalin on prevention and treatment of COPD. Key words: Baicalin; Chronic obstructive pulmonary disease;  Lung tissue; COPD model rats