Experimental studies on the relationship between nanobacteria translocation and SIRS of rats which suffering critical infection of abdominal cavity

Objective To explore the relationship between nanobacteria(NB) translocation from blood and stool and SIRS of rats suffering critical infection of abdominal cavity. Methods Forty SD rats were involved in the study. The models of critical abdominal cavity infection were made by cecal ligation plus puncture (CLP). The test samples of blood and stool were obtained before and two days after infected, respectively. The samples were cultured with NB culture medium and identified by transmission electron microscopy (TEM). The morphological change of the pathologic lesion in the ileum tissue of the rats were observed. Results Two days after infected, it showed epithelia abscission and tight junction loosening in some area of intestinal mucosa of the rats. Compared with preoperative, the average rectal temperature (℃) of the experimental group rats was higher significantly (39.98±0.43 vs 38.37±0.34, t = 18.538, P < 0.01), and the average numer of leukocyte (×109/L) was higher significantly(21.13±3.27 vs 10.43±2.23, t = 15.909, P < 0.01) at postoperative 2 d. The positive rates of NB were 17.5% and 42.5% (χ2 = 5.952, P = 0.015) in the blood samples before and after CLP 2 d respectively by TEM, and that were 90.0% and 92.5% (χ2 = 0.156, P = 0.692) in the stool. Conclusion The positive rates of NB increases in the blood samples, which may be associated with SIRS induced by critical abdominal cavity infection. Key words: Abdominal Cavity; Infection; Nanobacteria; SIRS