Sinosporangium fuscum sp. nov., isolated from soil
2015
A novel actinomycete, A-T 8343T was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343T belonged to the genus
Sinosporangium
and was closely related to
Sinosporangium siamense
A-T 1946T (98.81 %) and
Sinosporangium album
6014T (98.54 %). The DNA–DNA relatedness values were 21.8–27 % with
S. siamense
A-T 1946T and 31.1–31.9 % with
S. album
6014T, which were significantly below 70 %. The result differentiated A-T 8343T from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C16 : 0, iso C16 : 0, unsaturated C16 : 1 and C18 : 1. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus
Sinosporangium
to be named Sinosporangium
fuscum sp. nov. The type strain is A-T 8343T ( = BCC 52770T = NBRC 109516T).
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