Abstract 20282: Lipid Phosphate Phosphatase 3 and Lysophosphatidic Acid Receptors Expression in Human Atherosclerotic Plaques: Co-Localization of Relevant Lipid

Introduction: Single nucleotide polymorphisms (SNP) in the gene encoding lipid phosphate phosphohydrolase 3 (PPAP2B/LPP3) are associated with susceptibility to atherosclerotic cardiovascular disease (ACVD) independently of classical risk factor. LPP3 inactivates lysophosphatidic acid (LPA), a pro-atherogenic, -inflammatory and -thrombotic lipid generated by phospholipase activity. Thus, changes in PPAP2B/LPP3 arterial expression levels may be associated to ACVD. Hypothesis: PPAP2B/LPP3 and LPA receptors (LPAR1 to 6) differently expressed in human atherosclerotic lesions compared with normal arteries and co-localize with LPA Methods: Immunohistochemistry, in situ hybridization and microarray gene analysis were used to investigate expression. Lipid species were detected with in situ lipid mass spectrometry imaging. Gene silencing in human endothelial cell were performed to investigate LPA-induced activation. Results: PPAP2B/LPP3 mRNA was lower in carotid plaques compared to normal arteries. PPAP2B mRNA was ...