Effects of bile acids and acidic exposure on IL-8 expression in human esophageal epithelial cells

exposed on the surface of remaining tissue Primary squamous oesophageal epithelial cells (Sq cells) were isolated by explant tissue culture of normal oesophageal nmcosa. These cells were subsequently propagated in a low Ca + + serum-free tissue culture maintenance medium Sq cells were allowed to adhere to the luminal basement membrane of either Sq BM orCLO BM. Sq cells cultured in this orientation are at an airdiqind interface, which allow prolderation, stratification and dift?rentiation in response to the high Ca + + medium and interaction with the ECM and basement membrane. Results: The model was cultured for three weeks and histology confirmed sqnamous epithelium colonises sqnamous BM Laminin, an abundant ECM component was characterised by immunohistochemical staining. Conclusions: Tile role of the basement membrane in CLO on Sq cell proliferation and diflerentiation can be examined using this model