[Effect of Erxian Decoction on cyclophosphamide-induced oligospermia in mice].

: 目的： 探讨二仙汤对环磷酰胺致少精子症模型小鼠生殖功能的影响。方法： 雄性昆明小鼠随机分为正常组、模型组、二仙汤低/中/高剂量组等5组，每组16只小鼠。二仙汤低/中/高剂量组按体重每天分别灌胃3、6、12 g/kg的二仙汤，正常组和模型组则按体重灌胃相应体积生理盐水30d。从给药第21天起模型组、二仙汤低、中、高剂量组则同时腹腔注射环磷酰胺（80 mg/kg），正常组则腹腔注射生理盐水，连续5 d。在末次灌胃24 h后处死小鼠，取双侧附睾制备精子悬液，以随机盲测方式观察精子的数量和活力；取小鼠双侧睾丸，做睾丸组织切片，观察睾丸形态以及组织匀浆，以生化方法检测睾丸组织超氧化物歧化酶（SOD）、丙二醛（MDA）、谷光氨肽（GSH）的浓度。结果： 与正常组相比，模型组小鼠的附睾精子浓度［（9.31±1.32)×10⁶/ml vs (3.32±1.13）×10⁶/ml］与活力明显下降［（44.75±8.12)% vs (25.95±11.41）%］，SOD［(37.27±0.99) U/mg prot vs (14.23±1.99) U/mg prot］、GSH［(101.55±8.74) μmol/L vs (58.77±8.93) μmol/L］浓度降低(P<0.01)，MDA含量升高，组织切片显示管腔内精子的数量减少，生精细胞排列比较稀疏。而二仙汤具有明显改善作用，低、中、高剂量的二仙汤都能提高附睾精子浓度［(8.34±2.59)×10⁶/ml;(8.59±1.10)×10⁶/ml ;(8.41±1.47)×10⁶/ml ］、活力［(36.04±12.33)%；(38.87±13.13)%；(41.90±8.09)%］以及SOD［（22.99±1.11) U/mg prot；(20.82±1.81) U/mg prot；(21.33±1.66） U/mg prot］、GSH［（104.74±2.47) μmol/L；(98.61±12.98) μmol/L；(108.89±5.85） μmol/L］含量，降低MDA含量等。结论： 中药复方二仙汤能改善环磷酰胺致精子浓度减少和活力降低等病证，其作用机制可能与提高抗氧化能力，减少氧化应激损伤有关。. METHODS: Eighty 6-week-old male Kunming mice were randomly divided into five groups of equal number, normal control, OS model control, and low-, medium- and high-dose EXD, the former two groups treated intragastrically with normal saline and the latter three with EXD at 3, 6 and 12 g per kg of the body weight qd for 30 days. From the 21st day of administration, the mice of the normal control group were injected intraperitoneally with saline and those of the other four groups with cyclophosphamide at 80 mg per kg of the body weight qd for 5 consecutive days. At 24 hours after the last gavage, the bilateral epididymides of the mice were collected and sperm suspension prepared for determination of the sperm count and motility, and the bilateral testes were harvested for histomorphological observation and measurement of the concentrations of superoxide dismutase (SOD), malondialdehyde (MAD) and glutathione (GSH) in the testis tissue. RESULTS: Compared with the normal controls, the mice of the OS model control group showed significant decreases in epididymal sperm concentration (［9.31 ± 1.32］ vs ［3.32 ± 1.13］×107/ml, P <0.01) and motility (［44.75 ± 8.12］% vs ［25.95 ± 11.41］， P<0.01) and the concentrations of SOD (［37.27 ± 0.99］ vs ［14.23 ± 1.99］ U/mg prot, P <0.01) and GSH (［101.55 ± 8.74］ vs ［58.77 ± 8.93］ μmol/L, P <0.01) but an obvious increase in the MDA level (［2.21 ± 0.65］ vs ［2.61 ± 0.15］ nmol/mg prot, P <0.05) in the testis tissue. In comparison with the OS model controls, the mice treated with low-, medium- and high-dose EXD exhibited significantly increased epididymal sperm concentration (［8.34 ± 2.59］, ［8.59 ± 1.10］ and ［8.41 ± 1.47］×107/ml) (P <0.01) and motility (［36.04 ± 12.33］%, ［38.87 ± 13.13］% and ［41.90 ± 8.09］%) (P <0.01) and concentrations of SOD (［22.99 ± 1.11］, ［20.82 ± 1.81］ and ［21.33 ± 1.66］ U/mg prot) (P <0.01) and GSH (［104.74 ± 2.47］, ［98.61 ± 12.98］ and ［108.89 ± 5.85］ μmol/L) (P <0.01) but decreased level of MDA (P <0.05). CONCLUSIONS: Erxian Decoction can improve cyclophosphamide-induced reduction of sperm concentration and motility, which might be associated with its abilities of resisting oxidation and reducing oxidative stress injury.