Investigation of depressant effect of Yifei Yin on transplantation tumor of human lung adenocarcinoma A549 cells in nude mice and its related mechanism.

Objective:To observe the depressant effect of Chinese formula Yifei Yin on transplantation tumor of human lung adenocarcinoma A549 cells in nude mice,investigating the possible mechanism of the therapeutical effect of Yifei Yin on lung cancer.Methods:We inoculated the lung adenocarcinoma A549 cells on the nude mice to form transplantation tumor.The experimental nude mice with transplantation tumor were divided into five groups:negative control group,positive control group(oleum fructus bruceae group),high,moderate and low dose groups of Yifei Yin.Mice were intragastric administration and killed after 27 days.Transplantation tumor were taken and then detected.The cell cycle,CD95(Fas),Bcl-2 were detected by flow cytometry and Caspase-3,P16,VEGF,P53 were detected by immunohistochemical method,and the apoptotic index was detected by TUNEL method.Results:Moderate dose groups of Yifei Yin and positive control group can restrain the growth of the transplantation tumor obviously(tumor inhibitory rate 29.9%,35.9%),and increase the ratio of G0/G1 period(65.7%±20.7%,63.6%±27.3%).The expression of P53(4.55%±1.48%,4.67%±0.790%),P16(45.6%±19.5,51.4±29.1%),CD95(Fas),Caspase-3 of moderate dose groups of Yifei Yin and positive control group were up-regulated,and the expression of VEGF(20.9%±8.44%,21.3%±9.28%)and Bcl-2(2.51%±1.37%,2.34%±1.68%)of were both down-regulated.Apoptotic ratio(27.7%±8.60%,30.5%± 10.3%)and apoptotic index(1.53%±1.14%,1.47%±1.29%)of the two groups increased.The expression of CD95(Fas)(65.3%± 9.77%,70.4%±14.2%)and Caspase-3(10.0%±4.26%,9.61%±3.66%)of the two groups were both up-regulated also.Compared with negative control,the difference was all significant(P0.05).The effect of the moderate dose group of Yifei Yin and positive control group were similar(P0.05).Conclusion:Yifei Yin can restrain the transplantation tumor of human lung adenocarcinoma A549 cells in nude mice,and the mechanism may be related to inhibiting cell multiplication and inducing cell apoptosis.