High antioxidant activity of extracts obtained from sage by supercritical co2 extraction

The ethanolic extract of sage(Salvia officinalis L.) was separated into five fractions through reextraction with supercritical CO2. Further fractionation of the most active antioxidant fractions by means of liquid chromatography, with silicic acid as absorbent, yielded 2H-10,4α-(epoxy methano)-phenantren-12-one-1,3,4,9,10,10αhexahydro-5, 6-dihydroxy-9α-ethoxy-1,1-dimethyl-7-(1methylethy), (rosmanol-9-ethyl ether). The same compound was isolated from the alcoholic extract of the hyssop(Hyssopus officinalis L.). Rosmanol-9-ethyl ether was shown to be one of the active antioxidant components in sage and hyssop, with activity much greater than butylated hydroxytoluene (BHT).