D-FFOCT as a tool to assess oxygenation induced changes in cellular metabolism (Conference Presentation)

Dynamic Full-Field Optical Coherence Tomography (D-FFOCT) is a high transverse resolution version of OCT that records signal at the output of the interferometer as a movie over a few seconds. Analyzing the temporal variation of the signals reveals intracellular contrast which gives D-FFOCT the capability of identifying dynamic metabolic changes. We use D-FFOCT to identify oxygen induced changes in cellular metabolism at ambient (22%) oxygenation and at hypoxia (1%). Signal strength, calculated as an integral of the raw FFT spectrum, is significantly higher for 1% samples compared to 22% indicating that D-FFOCT is sensitive to changes in cell metabolism.