Neuroprotective effects of recombinant rAAV-AsiNOS on spinal cord ischemia

Objective To explore the neuroprotective effects of recombinant rAAV-AsiNOS transfection in vitroon spinal cord ischemia.Methods The spinal cord ischemic injury animal model was established by occlusion of the abdominal aorta.The spinal cord ischemic animal models were estimated by desending neurogenic motor evoked potentials,the ultrastructure of spinal cord after ischemic injury was observed under the light microscopy.The mRNA expression levels of iNOS,p38MAPK and Caspase-3 were semi-quantitatively detected and compared by using reverse transcription-polymerase chain reaction (RT-PCR) in G24,G48,G72,G120 groups and RG24,RG48,RG72 and RG120 groups.Results The amplitude pathological section of recombinant rAAV-AsiNOS transfection groups were gradually improvement,RT-PCR showed that there is significant difference between the rAAV-AsiNOS transfection groups and the ischemic groups for the expression of mRNA of iNOS,p38MAPK and Caspase-3.The relative values of three genes both 24 h in ischemic group (G24) and 24 h in ischemia group with viruses (RG24) were 0.45 and 0.25,0.56 and 0.27,0.50 and 0.24;and the relative values of both G120 and RG120 were 0.53 and 0.33,0.64 and 0.25,0.54 and 0.25.The expression of three genes in recombinant viral vectors group is significantly lower than those in the ischemic groups with the same time,and the difference was significant (P＜0.05 ).Conclusion The recombinant rAAV-AsiNOS transfection in vitro spinal cord ischemia can reduce the expression of mRNA of iNOS,p38MAPK and Caspase-3. Key words: Spinal cord ischemia;  iNOS;  AAV