UPLC: A PREEMINENT TECHNIQUE IN PHARMACEUTICAL ANALYSIS

The pharmaceutical companies today are driven to create novel and more efficient tools to discover, develop, deliver and monitor the drugs. In this contest the development of rapid chromatographic method is cru- cial for the analytical laboratories. In precedent decade, substantial technological advances have been done in enhancing particle chemistry performance, improving detector design and in optimizing the system, data processors and various controls of chromatographic techniques. When all was blended together, it resulted in the outstanding performance via ultra-high performance liquid chromatography (UPLC), which holds back the principle of HPLC technique. UPLC shows a dramatic enhancement in speed, resolution as well as the sensi- tivity of analysis by using particle size less than 2 µm and the system is operational at higher pressure, while the mobile phase could be able to run at greater linear velocities as compared to HPLC. This technique is con- sidered as a new focal point in field of liquid chromatographic studies. This review focuses on the basic prin- ciple, instrumentation of UPLC and its advantages over HPLC, furthermore, this article emphasizes various pharmaceutical applications of this technique.