High frequency of Helicobacter pylori in the esophageal mucosa of dyspeptic patients and its possible association with histopathological alterations

Summary Background Helicobacter pylori gastric colonization is known to be high in symptomatic subjects. However, only a few reports on the presence of H. pylori in the esophageal mucosa have been published. The aim of this study was to assess the frequency of H. pylori in the esophagus of dyspeptic patients and its association with histopathology. Methods The presence of H. pylori in the gastroesophageal mucosa was detected by fluorescence in situ hybridization (FISH) and PCR analysis of DNA extracted from gastric and esophageal biopsies of 82 symptomatic patients, using genus- and species-specific PCR primers. Alterations in the gastroesophageal mucosa were assessed by conventional histological techniques. Results H. pylori in the stomach was detected by PCR and FISH, respectively, in 61% ( n =43) and 90% ( n =63) of dyspeptic patients, and in the esophagus in 70% ( n =44) and 73% ( n =46). The prevalence of cagA -positive strains by PCR varied from 50% ( n =35) in the gastric mucosa to 65% ( n =41) in the esophageal mucosa. By combining the results of both methods, H. pylori was present in the gastroesophageal mucosa in 86% ( n =68) of patients. The association of the presence of bacteria, including H. pylori , in the esophageal mucosa with histopathological alterations was statistically significant between microabscesses and bacteria ( r =0.656, p r =0.25, p Conclusions This is the first report of the occurrence of H. pylori in the esophageal mucosa from dyspeptic Venezuelan patients. These results demonstrate the high prevalence of H. pylori in the esophagus, and its presence was correlated with signs of inflammation.