A microbiological culture comprising a hydrogel sheet polysaccharide dehydrated

The present invention belongs to the field of microbiology and culture of microorganisms. It relates more specifically to alternative solutions to conventional solid culture media. In this context, it proposes a microbiological culture device comprising: - a piece of absorbent material having an upper surface at least substantially flat and incorporating in its thickness, a composition of dehydrated culture medium, - based on said piece of absorbent material, a hydrogel sheet dehydrated and rehydratable polysaccharide at temperatures between 5 ° C and 40 ° C; said dehydrated hydrogel sheet is affixed directly to the upper face of said piece of absorbent material, or indirectly through an intermediate permeable membrane.