Rule of Intersection Point between Retention of Homologues and Column Temperature in Reversed-Phase Liquid Chromatography

The rule of intersection point between retention of homologues and column temperature in reversed-phase liquid chromatography is reported, based on the thermodynamic theory, with the basic equation of the retention in reversed-phase liquid chromatography, proposed by Vigh and others. We have derived the rule of intersection point between the logarithm of capacity factor and column temperature for homologues in reversed-phase liquid chromatography, and used the experimental data to verify it. The result is satisfactory.