Biocompatibility of human brain-derived neurotrophic factor gene-modified bone marrow mechanical stem cells and self-assembling peptide hydrogel in vitro
2017
Objective
To Investigate the biocompatibility of human brain-derived neurotrophic factor gene-modified bone marrow mechanical stem cells (hBDNF-rMSCs) and self-assembling peptide hydrogel in vitro.
Methods
hBDNF-rMSCs were successfully constructed using engineering technique in our former study. hBDNF-rMSCs were cultured in RADA16 self-assembling peptide hydrogel and functionalized RADA16-PRG self-assembling peptide hydrogel, as well as dulbeeeo modified eagle medium (DMEM) medium (control). Cell growth was detected using bromodeoxyuridine (BrdU) assay and cell proliferation was detected using methyl thiazol tetrazolium (MTT) assay in different group at different time.
Results
Cell growth and cell proliferation in RADA16-PRG group at different time were significantly increased comparing with control and RADA16 group (Pd1=0.000, Pd3=0.001, Pd5=0.000; Pd1=0.002, Pd3=0.019, Pd5=0.001). There were no significantly difference between control and RADA16 group in cell growth and cell proliferation at different time (Pd1=0.138, Pd3=0.063, Pd5=0.137; Pd1=0.240, Pd3=0.078, Pd5=0.054).
Conclusion
Both RADA16 self-assembling peptide hydrogel and functionalized RADA16-PRG self-assembling peptide hydrogel had good biocompatibility with hBDNF-rMSCs, it seemed that functionalized RADA16-PRG self-assembling peptide hydrogel could improve cell growth and cell proliferation of hBDNF-rMSCs.
Key words:
Human brain-derived neurotrophic factor; Bone mesenchymal stem cells; Self-assembling peptide; Hydrogel; Biocompatibility
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