Partial cloning and analysis of swine HEV isolates in Shanghai.

This study was to determine the condition of prevalence at several pig farms in Shanghai.We collected 36 swine fecal samples.Through the RT-PCR method,we amplified the partial ORF2 of HEV genome using univerisal primers.5 samples of 36 were positive for RT-nPCR,and the ratio of positive for RT-nPCR was 13.9 %.Taking out the same sequence,we got two partial sequences of HEV ORF2.The analysis of sequence suggests that the genetic distance between the two sequences and genotype I,II,III,IV were 0.183,0.207,0.230,0.102 and 0.177,0.226,0.240,0.108,respectively.Phylogenetic analysis indicated that both sequences belonged to genotype IV.