Pancreatic damage in obese mice with liver steatosis: effects of Ω 3 fatty acid (909.1)

Fatty liver disease (FLD) correlates with obesity and insulin resistance. The OB/OB leptin knock out mouse is used here to evaluate FLD, effects on the pancreas and the response to Ω 3 fatty acid (Ω3FA) treatment. The receptor for Ω3FA (Ω3FA-r) stimulates incretin which reduces inflammation, insulin resistance and type 2 diabetes. However, the Ω3FA-r has 2 isoforms, only one with activity. Our pilot studies showed elevated blood glucose in OB/OB mice following Ω3FA. Does this suggest an inactive receptor isoform in our model? Is it related to FLD or to pancreatic changes? OB/OB mice (10/group) were fed a standard or Ω3FA supplemented diet. Liver and pancreas were evaluated. Liver: Fat replaced about 70% of the parenchyma. Many hepatocytes contained pyknotic nuclei. Portal triads and biliary ducts were inflamed. Pancreas: Acinar structure disruption, necrosis, vasculitis and ductal inflammation were seen in the exocrine pancreas and peripancreatic fat but not in the endocrine pancreas. Blood glucose was si...