Application of micro-CT in small animal imaging.

Over the past decade, the number of publications using micro-computed tomography (μCT) imaging in preclinical in vivo studies has risen exponentially. Higher spatial and temporal resolution are the key technical advancements that have allowed researchers to capture increasingly detailed anatomical images of small animals and to monitor the progression of disease in small animal models. The purpose of this review is to present the technical aspects of μCT, as well as current research applications. Our objectives are threefold: to familiarize the reader with the basics of μCT techniques; to present the type of experimental designs currently used; and to highlight limitations, future directions, in μCT-scanner research applications, and experimental methods. As a first step we present different μCT setups and components, as well as image contrast generation principles. We then present experimental approaches in order of the evaluated organ system. Finally, we provide a short summary of some of the technical limitations of μCT imaging and discuss potential future developments in μCT-scanner techniques and experimental setups.