Abstract C63: The analysis of microbiota composition between tumor and normal tissues in pancreatic cancer and the comparison with the public data of NCBI

Purpose: The translocation of gut bacteria and its derivatives may be an essential factor contributing to the various diseases of the pancreas. However, microbial function on the development and progression of pancreatic cancer is not clear and the research is still lacking. The aim of this study is to analyze the composition of microbiota in pancreatic cancer tissue and to compare the result with the existing public data. Methods: We obtained the cancer and normal tissues from patients who underwent pancreatic cancer surgery in Korea (IRB: 2018-0710), and total bacterial genomic DNA was purified from tissue. Through the gene sequencing of 16S rRNA (V3-V4 lesion), we analyzed the quantitative and qualitative composition of microbiome. Then we compared the microbiome composition with public data of NCBI (SRP132007). Results: We successfully performed the gene sequencing of 16s rRNA from 12 tumor tissues and 6 normal tissues. Based on the results obtained from the tumor and normal tissues, we compared the composition from the phylum to the species levels. We confirmed that there were significant differences in the microbiome composition of the tumor and normal tissues and identified various strains of 20 or more species with a high specificity in tumor or normal tissues. In addition, through the comparison with SRP132007 data, we confirmed a distinct difference between the two groups. Conclusions: We have analyzed the patterns and characteristics of tumor or normal specific microbiomes through pancreatic cancer tissues. There is a clear difference between the two groups with environmental differences, even in the microbiomes of patients with the same disease. We expect to further clarify the importance of microbiomes in pancreatic cancer and point to the development of new therapies through further studies. Citation Format: Hwi Wan Choi, Jinju Kim, Eo Jin Kim, Eunsung Jun. The analysis of microbiota composition between tumor and normal tissues in pancreatic cancer and the comparison with the public data of NCBI [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer: Advances in Science and Clinical Care; 2019 Sept 6-9; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2019;79(24 Suppl):Abstract nr C63.