Screening effective differential expression genes for hepatic carcinoma with metastasis in the peripheral blood mononuclear cells by RNA-seq

// Yanting Shen 1, 2 , Lu Bu 3 , Rui Li 1 , Zhenzhu Chen 1 , Fei Tian 1 , Na Lu 2 , Qinyu Ge 2 , Yunfei Bai 2 , Zuhong Lu 1, 2 1 Research Center for Learning Science, Southeast University, Nanjing, Jiangsu Province 210096, PR China 2 State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu Province 210096, PR China 3 Department of Interventional Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, Jiangsu Province 210009, PR China Correspondence to: Qinyu Ge, email: geqinyu@seu.edu.cn Keywords: hepatic carcinoma, metastasis, PBMC, tumor heterogeneity, diagnosis Received: December 06, 2016      Accepted: February 20, 2017      Published: March 02, 2017 ABSTRACT Tumor metastasis is a multistep process involving a number of genetic alterations so that the genetic diagnosis is got increasingly attentions today. The aim of this study was to use RNA-seq to screen the effective differential expression genes in the peripheral blood mononuclear cells for the hepatic carcinoma with metastasis. The results showed that hepatic carcinoma samples gathered according to different metastasis. CCL3 , CCL3L1 , JUN , IL8 , and IL1B were identified in inflammation mediated by chemokine and cytokine signaling pathway (P00031) in the hepatic carcinoma samples with metastasis, and subsequently confirmed by quantitative real-time polymerase chain reaction. In conclusions, CCL3 , CCL3L1 , JUN , IL8 , and IL1B have the potential to be considered as candidates for future molecular diagnosis of the hepatic carcinoma with metastasis. This work may provide us with new visions into the metastasis process and potential efficient clinical diagnosis in the future.