Screening and optimization of Halophilic strains for Polyhydroxybutyrate production isolated from Sambhar Lake, Rajasthan

Poly-β-hydroxybutyrate (PHB) is gaining importance in advanced material research through its application in the field of bioplastic material and medical field applications. Production of PHB has been optimized in bacterial species. The current work focuses on isolation characterization of halophilic bacterial strain from Saltern Sambhar lake, Rajasthan and optimization for high production of PHB. The optimized growth was studied in different media Nutrient broth, M9, HSM and GM 2. Modified DSC-97. Further optimization studies were carried out in different pH (7-10), sodium chloride concentrations (1-5 M) incubation time (24, 48, 72, 96 and 120 hrs) and variable carbon sources (glucose 1-4%). PHB was extracted by chloroform extraction protocol and was detected and characterized by TLC and FTIR. Halophilic bacterial strain was identified through 16s RNA sequencing method.