Effects of liquid digestate on the valence state of vanadium in plant and soil and microbial community response.

Abstract Liquid digestate containing high levels of nutrients and humic and fatty acids can affect vanadium species and their plant uptake. To elucidate the effects of liquid digestate on the valence state of vanadium in soil and plant tissue, as well as its effects on the microbial community and soil properties, we grew green bristlegrass (Setaria viridis), a native plant capable of growing in vanadium mining areas, in vanadium-contaminated soils sampled from a mining area and treated it with 5% and 10% liquid digestate for 90 d, respectively. Changes in the concentrations of pentavalent (V[V]) and tetravalent (V[IV]) vanadium in the soils and the shoots and roots of bristlegrass and the soil microbial abundance were measured. The results showed that vanadium existed mainly in the form of V(IV) in the soil but accumulated mainly in the form of V(V) in the bristlegrass. Liquid digestate markedly reduced V(V) concentrations in the soils (by up to 45%) and in the shoots and roots of green bristlegrass (by up to 98%). Liquid digestate enhanced the abundance of Bacteroidetes, which can reduce V(V) to lower valence state. Microbial reduction and phosphorus immobilization were responsible for downregulating V(V) concentrations in the plant and soil. The liquid digestate can be used to enhance in situ bioremediation of vanadium-contaminated soil in mining area.