Effect of tetramethylpyrazine derivative H_(168) on hepatic stellate cells proliferation and its possible mechanism

Aim To observe the effect of tetramethylpyrazine derivative H168 on hepatic stellate cells(HSC) proliferation and its possible mechanism.Methods MTS was used to detect the effect of tetramethylpyrazine derivative H168 on HSC-T6 proliferation.Cell cycle was measured by flow cytometry(FCM) analysis with PI dual staining.Western blotting and Real time PCR were used to detect the protein and mRNA expression of p21/p27.Results Tetramethylpyrazine derivative H168 inhibited the proliferation of HSC-T6 obviously.With the increase of drug concentration,the potential efficacy on inhibition of HSC-T6 proliferation showed a dose-dependent manner.Cycle detected by flow cytometry of the drug showed the cells accumulated in G0/G1 phase,and reduced in S and G2/M phases.FCM detection found tetramethylpyrazine derivative H168 with the dose-effect promotion of apoptosis on HSC.Western blot results showed the tetramethylpyrazine derivative H168 inhibited HSC-T6 proliferation by promoting p21/p27 protein expression.Further use of Real time PCR found that ligustrazine derivative H168 promoted expression of p21/p27 mRNA.Conclusion H168 can inhibit HSC-T6 proliferation mainly through promoting the protein and mRNA expression of p21/p27.