[Relationship between the expression of matrix metalloproteinase-13 protein and other biomarkers, prognosis in invasive breast cancer].

Objective The study was designed to investigate the expression patterns ofmetalloproteinase (MMP)-13 protein in invasive breast carcinoma and to determine the clinicopathologicaland prognostic values of its various localization and relation to the tumor phenotypes. MethodsImmunohistochemistry was performed on paraffin-embedded tissue array from 263 invasive breast carcinomasto investigate the protein expressions of MMP-13, estrogen receptor, progesterone receptor, HER2, MMP-2,MMP-9 and tissue inhibitor of matrix metaUoproteinases (TIMP) -1, TIMP-2. Results MMP-13 protein wasdetected in the cytoplasm of carcinoma cells and peritumoral fibroblasts. High level expression of MMP-13protein in tumor cells was associated with more lymph node involvement and higher tumor grade (both P <0. 01), and positively correlated with HER2 (P = 0. 015) and TIMP-1 protein (P < 0. 01) expression incarcinoma cells. Moreover, high expression of MMP-13 was associated with shortened overall survival for theentire patient population and the patient group with positive lymph node. Tumor cell derived MMP-13 haddifferent impact on patients with different HER2 status. Peritumoral fibroblasts derived MMP-13 protein,although correlated with tumor cell derived MMP-13 and associated with lymph node stage and HER2expression, was found having less prognostic impact. Univariate survival analysis showed that the tumor size,grade, lymph node status, PR status, HER2 expression, tumors TIMP-1 and MMP-13 expression wereprognostic factors. However, multivariate survival analysis showed that only tumor size, lymph node status,HER2 expression, tumors TIMP-1 and MMP-13 were independent prognostic factors. Conclusion MMP-13protein expressed by tumor cells correlates with the invasion and metastasis of breast carcinoma, andtherefore, may serve as a poor prognostic marker for the patient. Key words: Breast neoplasms;  Matrix metalloproteinase 13 ;  Microarray analysis;  Prognosis