Nano-Rods Structured Cerium Oxide Platform for Cholesterol Biosensor

This study presents a cholesterol biosensor that uses cerium oxide nanorods (CeO2-NRs) as a platform for cholesterol oxidase (ChOx) attachment. The properties of the synthesized CeO2-NRs were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM). The ChOx/CeO2-NR-modified electrode response displayed a high sensitivity of 85.8 μA mM−1 cm−2 over a linear detection range between 1 and 6.5 mM of cholesterol concentration and a limit of detection of approximately 0.68 mM. Optimal parameters, including pH value, temperature, and amount of ChOx, as well as the reproducibility, specificity, and stability of the ChOx/CeO2-NR-modified electrode, were also investigated.