Isolation of Neolignans from Ocotea elegans by CCC

Abstract Countercurrent chromatography was used for the separation of neolignans from stems of Ocotea elegans. This separation was performed using a clean up system as prefractionation and the concentrated neolignans were submitted to CCC using Hexane:EtOAc:MeOH:H2O (1:2:2:1 v/v/v/v) as the solvent system. It was possible to isolate burchellin, guaianin, and canelin, showing the high efficiency of CCC for the neolignan mixture separation.